Plasmid

Part:BBa_M39203:Design

Designed by: Hannah Kempton   Group: MIT BE 20.20-S12   (2012-05-15)


Vector for creation of micro-dystrophin SFV


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2190
    Illegal PstI site found at 2949
    Illegal PstI site found at 3213
    Illegal PstI site found at 3312
    Illegal PstI site found at 3778
    Illegal PstI site found at 4149
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2190
    Illegal NheI site found at 1337
    Illegal PstI site found at 2949
    Illegal PstI site found at 3213
    Illegal PstI site found at 3312
    Illegal PstI site found at 3778
    Illegal PstI site found at 4149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2190
    Illegal BglII site found at 1483
    Illegal BglII site found at 1697
    Illegal BglII site found at 2607
    Illegal BamHI site found at 228
    Illegal BamHI site found at 2743
    Illegal BamHI site found at 3448
    Illegal XhoI site found at 2794
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2190
    Illegal PstI site found at 2949
    Illegal PstI site found at 3213
    Illegal PstI site found at 3312
    Illegal PstI site found at 3778
    Illegal PstI site found at 4149
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2190
    Illegal PstI site found at 2949
    Illegal PstI site found at 3213
    Illegal PstI site found at 3312
    Illegal PstI site found at 3778
    Illegal PstI site found at 4149
    Illegal NgoMIV site found at 4329
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2252
    Illegal SapI.rc site found at 2396


Design Notes

We had to make sure only to include the packaging sequences of SFV, so that it would not be able to replicate within the patient's cells. This should also reduce the virulent capabilities of the virus.


Source

This part came from a combination of a portion of the SFV genome, in addition to the micro-dystrophin genomic sequence and standard promoters and terminator.

References